Rapid versus standard antibiotic susceptibility testing for treating bloodstream infections

A multiplexed microfluidic platform for rapid antibiotic susceptibility testing.

Effective treatment of clinical infections is critically dependent on the ability to rapidly screen patient samples to identify antibiograms of infecting pathogens. Existing methods for antibiotic susceptibility testing suffer from several disadvantages, including long turnaround times, excess sample and reagent consumption, poor detection sensitivity, and limited combinatorial capabilities. Un...

Standard versus biofilm antimicrobial susceptibility testing to guide antibiotic therapy in cystic fibrosis.

BACKGROUND The antibiotics used to treat pulmonary infections in people with cystic fibrosis are typically chosen based on the results of antimicrobial susceptibility testing performed on bacteria traditionally grown in a planktonic mode (grown in a liquid). However, there is considerable evidence to suggest that Pseudomonas aeruginosa actually grows in a biofilm (or slime layer) in the airways...

Rapid Phenotypic Antibiotic Susceptibility Testing Through RNA Detection

Rapid Cytometric Antibiotic Susceptibility Testing Utilizing Adaptive Multidimensional Statistical Metrics

Flow cytometry holds promise to accelerate antibiotic susceptibility determinations; however, without robust multidimensional statistical analysis, general discrimination criteria have remained elusive. In this study, a new statistical method, probability binning signature quadratic form (PB-sQF), was developed and applied to analyze flow cytometric data of bacterial responses to antibiotic exp...

Antibiotic Susceptibility Testing

12.2 TERMINOLOGY Selectivity All Clinically effective antimicrobial agents exhibit selective toxicity towards the bacterium rather than the host. It is this characteristic that distinguishes antibiotics from disinfectants. The basis for selectivity will vary depending on the particular antibiotic. When selectivity is high the antibiotics are normally non toxic. However, even highly selective an...